Isolating the effects of individual particle properties (e.g. shape, size, mineralogy, surface roughness) on the mechanical behavior of naturally occurring coarse-grained soils is a significant challenge in experimental studies. This challenge can be addressed by recent advances in 3D printing technology which enable generation of artificial sand-sized particles with independent control over particle size and shape. In this study, bender element tests are conducted to examine the isolated effects of particle shape on the shear wave velocity and shear modulus of 3D printed sand analogs. The experimental results show that the shear wave velocity and shear modulus of the 3D printed sand specimens exhibit a relationship with mean effective stress that is in agreement to that reported for natural sands. The specimens composed of 3D printed sands with greater particle roundness and sphericity exhibit greater shear wave velocity and shear modulus for a given void ratio, relative density, and mean effective stress. The changes in shear wave velocity can be captured in terms of differences in individual particle shape parameters such as roundness and sphericity as well as combined particle shape parameters such as regularity. Regression analysis is used to develop relationships between shear wave velocity and particle shape parameters and void ratio, which are shown to be in agreement with previously-published relationships and to reliably predict the shear wave velocity of natural sands. The results presented herein highlight the usefulness of testing 3D printed soils to identify functional trends and dependencies between soil response parameters and intrinsic properties. However, this requires verification of the results against published trends and assessment of the possible effects of the differences in constituent material between the 3D printed and the natural soils.